Videos

The world's First Live-Cell Imaging

The world's first Live-Cell imaging (time-lapse cine photography) by Dr. Warren Harton Lewis in 1931. An isolated mouse macrophage induces macropinocytosis.

A COS7 Cell Infected by Shigella Flexneri

Time-lapse images of a COS7 cell infected by Shigella flexneri (Yohisda et al., Science 2006). Shigella can move in the infected cells by modulating the host cytoskeleton mechanism.

Macrophage Macropinocytosis

Time-lapse images of macrophage macropinocytosis. After macrophage-colony stimulating factor (MCSF), the cells induce membrane ruffles, some of which convert to macropinocytosis.

Comparison of Phage and Ratio Images

Comparison of phage (left) and ratio (right) images. As a probe protein to identify PIP3, YFP-BtkPH (and CFP as the reference) is expressed in macrophages. The cells were stimulated with MCSF. The merged image (middle) shows that PIP3 is transiently generated during macropinocytosis (red color in the image).

Live-Cell Imaging of "Squidlysis"

Tubular lysosomes and macropinosomes were stained by TRDx (Bottom-Right) and LY (Bottom-Left), respectively. Merge image (Middle-LEFT) shows that tubular lysosomes (as black in the image) elongate the structure to fuse with macropinosomes (white).

Live-Cell Imaging of MEFs Expressing YFP–Btk-PH and CFP after Stimulated by Growth Factor

Live-cell imaging of MEFs expressing YFP–Btk-PH (to detect PIP3) and CFP (as the reference of YFP signal) after stimulation by growth factor (JCS 2018). Growth factor induces "crater-like" structures called Circular Dorsal Ruffles (CDRs). Comparison of phase-contrast (left) and ratio (YFP–BtkPH/CFP) (right) images shows sustained PIP3 signal inside CDRs.

Live-Cell Imaging of MEF after Growth Factor Stimulation

Live-cell imaging of MEF. After growth factor treatment, circular dorsal ruffles(CDRs) are induced and then shrink to small bubbles called macropinosomes.

To be continued...